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BRET Filters
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Due to low signals BRET and other colour luminescence applications need an extremely efficient optical system together with appropriate filters as present in the Mithras or TriStar.
Berthold Technologies offers the following BRET filters. All filters have been tested extensively:
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Method |
Wavelength [nm] |
Order number |
Wavelength [nm] |
Order number |
| Standard filters |
BRET 1 |
480/20 |
39450 |
530/25 |
39451 |
| BRET 2 |
400/70 |
39448 |
515/20 |
39449 |
| High efficiency filters |
BRET 1 |
480/20 |
53425 |
540/40 |
53426 |
| BRET 2 |
410/80 |
53427 |
515/40 |
53428 |
The four standard BRET filters can be ordered as BRET package with order number 39350. Various Mithras models have the package already included.
The use of the high efficiency filters is recommended when either expression levels are low or when energy transfer has low efficiency.
Filter comparison
Together with Ralf Jockers (Institute Cochin/INSERM, U567, Paris, France) the efficiency of the high efficiency BRET 1 filters versus the standard BRET 1 filters have been compared.
The classical BRET donor Rluc and BRET acceptor YFP and the new optimized Rluc8 and YPet variants were used (Kamal et al., 2009).
The results clearly show that the optimized filter settings provide significantly improved BRET signals for all donor/acceptor pairs. BRET values determined with the high efficiency filter selection were approximately 50% higher for each donor/acceptor combination tested (see detailed application note).
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Download the detailed application note:
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